Catalog # 222030 – 30 tests

Description

Direct immunofluorescence assay for the detection of Pneumocystis carinii cysts and trophozoites in direct smears of respiratory tract specimens, based on monoclonal antibodies.

Features

-          Direct IFA requiring one single incubation

-          Detects both trophozoite and cyst stage

-          Monoclonal antibodies provide high sensitivity and specificity

Specimens

Bronchoalveolar lavage, bronchial wash and induced sputum.

Kit Components

Detection reagent, mounting medium, microscope slides and transfer pipets..

Assay Protocol:

1.    Air dry specimen on slide and fix in acetone for 10 minutes

2.    Place 50 mcl of detection reagent on each well and incubate for 30 minutes at 37°C.

3.    Wash with water.

4.    Add mounting medium and coverslip to the slide

5.    Examine the slide using a fluorescence microscope at 200-300x.

Storage

The kit must be stored at 2-8°C.

Additional Information

Click here for more information (complete procedure, safety data sheet, literature).