Catalog # 4873171 – 100 tests

Description

Indirect immunofluorescence assay for the semi-quantitative detection of antibodies directed against Legionella pneumophila serogroups 1 to 8 in human serum.

Features

-          Detects IgG, IgM, and IgA

-          Rapid procedure : 2 x 30 minutes

-          Two separate serogroup pools (1-4-6-8 and 2-3-5-7) for initial serotyping

-          Full kits and components available separately

Specimens

Serum.

Kit Components

10 slides (10 wells each) covered with a mix of inactivated Legionella bacteria (upper row: serogroups 1-4-6-8 - lower row serogroups 2-3-5-7), Negative control, Positive control, Sample diluent, Conjugate, Mounting medium, PBS buffer.

Assay Protocol:

1.    Dilute PBS buffer, and solve the sample diluant in PBS buffer.

2.    Dilute the enzyme conjugate and the 2 controls in sample diluent.

3.    Dilute the patient samples in sample buffer, at 1:128 for a screening, or serial dilutions for titration.

4.    Add 1 drop (15 µl) of diluted samples and control to the appropriate wells, and incubate at 37°C in wet room for 30 minutes.

5.    Wash the slides with diluted PBS and with distilled water.

6.    Place the slides in a wet room, add 15 µl of conjugate to each well, and incubate at 37°C in wet room for 30 minutes.

7.    Wash the slides with diluted PBS and with distilled water.

8.    Add mounting medium and coverslip to the slide.

9.    Examine the slide using a fluorescence microscope.

Storage

The kit must be stored at 2-8°C.

Additional Information

Click here for more information (complete procedure, safety data sheet, literature).