Catalog # 500112 – 40 tests

Description

Direct fluorescent technique designed for qualitative detection of Respiratory Syncytial Virus (RSV), in smears of nasopharyngeal cells or in cell culture.

Features

-          Use of 13-1 and 25-2 monoclonal antibodies conjugate.

-          Similar procedure to Merifluor FA kits.

Specimens

Nasopharyngeal specimens from aspirates, swabs or from cell culture isolates.

Kit Components

-          Phosphate-buffered Fluorescein-labeled conjugate containing Evans blue counterstain.

-          PVA-based mounting medium.

Specimen preparation

Specimens collected by aspiration or obtained with a swab are suspended in 2-3mL PBS. Cells from culture are removed by trypsinization or scraping.

-          Centrifuge obtained specimen (450g, 10min) and discard supernatant.

-          Re-suspend pellet in 1-2 drops of PBS.

-          Drop 20-40µL of the re-suspended pellet onto slide. Air dry.

-          Fix with cold acetone for 10min. Air dry.

-          Proceed within 2 hours or store slide desiccated at –20°C.

Assay Protocol:

1.       Add 1-3 drops of conjugate to specimen well.

2.       Incubate at room temperature in a moist chamber for 30min.

3.       Rinse carefully slides with a PBS solution.

4.       Add permanent mounting medium and apply a cover slip on the slides.

5.       Read slides using a fluorescence microscope.

Storage

The kit must be stored at 2-8°C.

Additional Information

Click here for more information (complete procedure, safety data sheet, literature).