Catalog # 500113 –  60 tests

Description

Direct fluorescent technique designed for qualitative detection of varicella-zoster virus (VZV), in vesicle smears and biopsy specimens.

Features

-          Use of monoclonal antibody conjugate.

-          Similar procedure to Merifluor FA kits.

Specimens

Smear, tissue culture, biopsy specimens.

Kit Components

-          Phosphate-buffered Fluorescein-labeled conjugate containing Evans Blue counterstain.

-          Buffered mounting medium containing photobleach inhibitor.

Specimen preparation

Prepare specimens as follows:

- Smear specimen: Spread collected cellular material on slide and air dry.

- Tissue culture specimen: Cells demonstrating VZV cytopathic effect are dropped onto slide.

- Biopsy Specimen: Frozen biopsy specimens are sectioned and air dried.

Fix obtained specimen with cold acetone for 10min. Air dry.

Proceed within 2H or store slide desiccated at 2-8°C.

Assay Protocol:

1.       Add 1-3 drops of conjugate to specimen well.

2.       Incubate at room temperature in a moist chamber for 30min.

3.       Rinse carefully slides with a PBS solution.

4.       Add mounting medium and apply a cover slip on the slides.

5.       Read slides using a fluorescence microscope.

Storage

The kit must be stored at 2-8°C.

Additional Information

Click here for more information (complete procedure, safety data sheet, literature).