Catalog # 602096 – 192 tests

Description

Microplate Enzyme-Immunoassay, based on monoclonal antibodies,  for the qualitative and semi-quantitative detection of capsular polysaccharide antigens of Cryptococcal neoformans in serum and cerebrospinal fluid.

Features

-        Superior sensitivity compared to latex tests

-        Simple 30 minutes procedure

-        No need for specimen pre-treatment

-        Break-away microwell strips

-        Qualitative and quantitative procedure: ideal for therapy monitoring

Specimen

Serum and Cerebrospinal Fluid

Kit Components

Conjugate, wash buffer, substrate, stop solution, positive control, sample diluent, Antibody coated microwells, microwell Strip Holder.

Assay Protocol:

1.       Transfer 50µL of specimen, negative control and positive control in separate microwells

2.       Incubate for 10 minutes at Room Temperature, then wash three times

3.       Add 1 drop of enzyme conjugate to each microwell

4.       Incubate for 10 minutes at Room Temperature, then wash three times

5.       Add 2 drops of substrate solution to each microwell

6.       Incubate for 10 minutes at Room Temperature

7.       Add 2 drops of stop solution to each microwell

8.       Read after 2 minutes at 450 nm or visually

Storage

The kit must be stored at 2-8°C.

Additional Information

Click here for more information (complete procedure, safety data sheet, literature).