Catalog # 616096 – 96 tests

Description

Microplate Enzyme-Immunoassay for the qualitative detection of Clostridium difficile Toxin A and / or Toxin B in human stools.

Features

-          Increased sensitivity with the detection of toxin A and toxin B

-          Fast procedure: 60 minutes or 30 minutes, if using a shaking incubator.

-          Break-away microtiter strips

-          Color coded ready-to-use reagents

-          Visual or spectrophotometer reading

-          1 positive + 1 negative controls per run

Specimen handling

The stool specimen, collected in an empty container, can be stored up to 3 days at 2-8°C before testing.

If testing is not performed within this time frame, specimens should be frozen upon receipt (-20°C to -80°C).

Kit Components

Antibody coated microwells, Positive control, Negative control, Sample diluent, Wash buffer, Enzyme conjugate, Substrate, Stop solution, Transfer pipets, Strip holder, Strip sealer.

Assay Protocol:

1.    Emulsify 5-6 mm diameter portion of stool into 200 µl of sample diluent in a test tube, or add 50 µl of liquid stool to 200 µl of sample diluant.

2.    Transfer 100 µl (2 drops) of diluted stools and controls in the appropriate wells.

3.    Add 1 drop of enzyme conjugate to each well, seal and incubate at 37°C for 50 minutes, or at 37°C under agitation for 20 minutes.

4.    Wash 7 times with the wash buffer.

5.    Add 2 drops of substrate to all wells, seal and incubate at room temperature for 10 minutes.

6.    Add 2 drops of stop solution, wait for 2 minutes and read at 450 nm or visually.

Storage

The kit must be stored at 2-8°C.

Additional Information

Click here for more information (complete procedure, safety data sheet, literature).