Catalog # 618096 – 96 tests
Description
Premier
Campy is an in vitro qualitative
ELISA
test for the detection of specific Campylobacter
antigens in stool samples from patients with signs and symptoms of
gastroenteritis. Premier
Campy detects C. jejuni and C. coli in human stool that may be either
unpreserved or preserved
in Cary Blair-based transport media.
Features
- Monoclonal antibodies- Break-away microtiter strips
- Colour coded ready to use reagents
- Room temperature incubations – shaking optional
- 1 Positive Control and 1 Negative Control per run – provided in the kit
- Time for the Test: 1 h 10 min – 1 h 40 min (shaking incubation procedure)
- Visual and spectrophotometer reading (450 nm or 450/630 nm)
Specimen handling
Human
stool sample that may be either unpreserved or preserved in Cary
Blair-based transport media, such as Meridian ParaPak C&S
(900612 – 120 vials).
Samples should be stored at 2–8 C prior to testing. Samples
should be tested as soon as possible, but may be held up to 96 hours at
2–8 C. Samples that will not be tested within 96 hours should
be frozen immediately upon receipt and stored at ≤ –20 C until tested.
Kit Components
Antibody coated microwells, Positive control, Negative control, Sample diluent, Wash buffer, Enzyme conjugate, Substrate, Stop solution, Transfer pipets, Strip holder, Strip sealer.
Assay Protocol:
1. Emulsify 3-4 mm diameter portion of stool into 200 µl of sample diluent in a test tube, or add 50 µl of liquid stool to 200 µl of sample diluent.
2. Transfer 100 µl of diluted stools and controls in the appropriate wells.
3. Incubate one hour at 21-27°C (30 minutes with shaking incubation)
4. Wash 5 times with the wash buffer.
5. Add 2 drops of enzyme conjugate to each well, seal and incubate at 21-27°C for 30 minutes.
6. Wash 5 times with the wash buffer.
7. Add 2 drops of substrate to all wells, seal and incubate at room temperature for 10 minutes.
8. Add 2 drops of stop solution and read at 450 nm, 450/630 nm or visually.
Storage
The kit must be stored at 2-8°C.
Additional Information
Click here for more information (complete procedure, safety data sheet, literature).
back