Catalog # 696007 – 48 tests

Description

Microplate Enzyme-Immunoassay for the qualitative detection of human Adenoviruses direct from stool specimens, and to confirm the presence of adenovirus in cell culture isolates from respiratory, ophthalmic or enteric specimens.

Features

-          Detection of the 41 serotypes of human adenovirus

-          Break-away microtiter strips

-          Room temperature incubations

-          Visual or spectrophotometer reading

Specimen handling

The stool specimen, collected in an empty container, can be stored up to 3 days at 2-8°C before testing.

If testing is not performed within this time frame, specimens should be frozen upon receipt (-20°C to -80°C).

For cell culture, all specimens should be held at 2-8°C on ice and not frozen unless a delay of more than 72 hours before cell culture inoculation is anticipated. In such cases, rapidly freeze the specimen in a bath of dry ice and acetone or alcohol, and store at –20°C or colder for up to one month.

Kit Components

Group specific anti-Adenovirus antibody coated microwells, Positive control, Sample diluent / Negative control, Enzyme conjugate, Part A substrate, Part B substrate, Stop solution, Transfer pipets, Strip holder.

Assay Protocol:

1.    Stool sample : with the transfer pipet, add a portion of stool into 1 ml of sample diluent in a test tube.

2.    Transfer 2 drops (100 µl) of diluted stools, and transfer 2 drops (100 µl) of undiluted tissue culture fluid and controls in the appropriate wells.

3.    Add 2 drops (100 µl) of enzyme conjugate to each well, mix and incubate at room temperature for 60 minutes.

4.    Wash 5 times with deionized water.

5.    Add 2 drops (100 µl) of substrate A to each microwell.

6.    Add 2 drops (100 µl) of substrate B to each microwell, and incubate at room temperature for 10 minutes.

7.    Add 2 drops (100 µl) of stop solution, and read at 450 nm or visually.

Storage

The kit must be stored at 2-8°C.

Additional Information

Click here for more information (complete procedure, safety data sheet, literature).