Catalog # 709030 – 30 tests

Description

Membrane EIA single test for the qualitative detection of IgM antibodies directed against Mycoplasma pneumoniae in human serum.

Features

-          Rapid detection : results within 10 minutes

-          Easy and single test procedure

-          Ready-to-use reagents in droppers

-          Internal control of the procedure on each card

Specimens

Serum.

Kit Components

Test cards, Positive control, Negative control, Enzyme conjugate, Wash buffer, Substrate, Transfer pipets.

Assay Protocol:

1.    Add 2 drops of sample or controls to the 2 sample wells (lower wells) on separated cards, and incubate 2 minutes at room temperature until complete absorption.

2.    Add 3 drops of enzyme conjugate to the 2 sample wells (lower wells) on each card, and incubate 2 minutes at room temperature until complete absorption.

3.    Add 3 drops of wash buffer to the 2 reaction wells (upper wells) on each card.

4.    Wait for the complete absortion, and add 2 drops of substrate to the 2 reaction wells (upper wells) on each card.

5.    Incubate at room temperature for 5 minutes, and read the result in the reaction wells (upper wells).

Interpretation of results

Positive result : blue color in the 2 reaction wells (Control + Test).

Negative results : blue color in the Control reaction well only.

Storage

The kit must be stored at 2-8°C.

Additional Information

Click here for more information (complete procedure, safety data sheet, literature).