ImmunoCard Toxins A & B
  Catalog # 712050 – 50 tests

Description
Membrane EIA single test for the qualitative detection of Clostridium difficile Toxins A and B in Stool Specimens.

Features
  • High sensitivity: both Toxin A and Toxin B are detected
  • Rapid detection: results in 15 minutes
  • No sample pre-treatment required
  • No reagent dilution required
  • Easy and single test procedure
  • Internal control of the procedure on each card

Specimen handling
The stool specimen, collected in an empty container, can be stored up to 3 days at 2-8°C before testing.
If testing is not performed within this time frame, specimens should be frozen upon receipt (-20°C to -80°C).

Kit Components
Test cards, Specimen Diluent, Enzyme Conjugate, Positive control, Wash Reagent, Substrate Reagent, Transfer pipettes.

Assay Protocol:

  1. Add 200 µl of Specimen Diluent to a test tube using the dropper in the bottle.
  2. Add 3 drops of Enzyme Conjugate to the test tube.
  3. Dispense 25 µl of stool (mixed thoroughly) or control into the Diluent/Conjugate mixture.
  4. Vortex the final mixture for 10 seconds. 
  5. Incubate at room temperature for 5 minutes.
  6. Add 150 µl of specimen to the 2 sample (lower) ports on one test card.
  7. Incubate at room temperature for 5 minutes.
  8. Add 3 drops of Wash Reagent in the 2 reaction (upper) ports.
  9. When the Wash Reagent has been completely absorbed, add 3 drops of Substrate in the 2 reaction (upper) ports.
  10. Incubate at room temperature for 5 minutes.
  11. Read the results in the reaction (upper) ports within 30 seconds of the end of incubation.

Interpretation of results
Positive result : blue color in the 2 reaction ports (Control + Test).
Negative results : blue color in the Control reaction port only.

Storage
The kit must be stored at 2-8°C.

Additional Information

Click here for more information (complete procedure, safety data sheet, literature).

 
  back
home