Catalog # 756500E – 100 tests
Description
Rapid Elisa test for serological diagnosis of Epstein-Barr virus infection in human serum or plasma, based on the detection of EBNA-1 IgM and IgG antibodies. The test uses a synthetic peptide corresponding to the EBNA-1 sequence P62.
Features
- Rapid detection : results within 10 minutes
- Easy and unitary procedure
- Visual interpretation of results
- Detection of specific antibodies of EBV infection
Specimens
Serum or plasma.
Kit Components
Peptide-coated paddles, IgM Enzyme conjugate, IgG Enzyme conjugate,
Substrate 1, Substrate 2, Substrate mixing vial, Wetting agent / wash solution, Stop solution, Paddle storage bag. Positive and negative controls available separatly.
Assay Protocol:
1. Transfer 50 µl of diluted wash solution to both wells of the appropriate number of paddles.
2. Add 75 µl of each patient specimen and one drop of controls to both wells of the appropriate paddle.
3. Incubate all paddles for 2 minutes at room temperature.
4. Rinse and wash the paddles 2 times with wash solution.
5. Add two drops of IgM enzyme conjugate to all the outer wells labeled M, and 2 drops of IgG enzyme conjugate to all the inner wells labeled G.
6. Incubate all paddles for 2 minutes at room temperature.
7. Rinse and wash the paddles 2 times with wash solution.
8. Add 2 drops of mixed substrate 1 + substrate 2 to both wells of each paddle.
9. Incubate all paddles for 2 minutes at room temperature.
10. Add 1 drop of stop solution to both wells of all paddles, mix, and read the results after 10 minutes.
Interpretation of results
A positive result will produce a green color in the paddle: compare the color intensity between the M and G wells. If the M well is higher than the G well, it is an acute infection. If the M well is equal or lower than the G well, it is an old infection.
Storage
The kit must be stored at 2-8°C.
Additional Information
Click here for more information (complete procedure, safety data sheet, literature).
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