Catalog # EA101 - 100 tests

Description

Indirect immunofluorescence assay designed for qualitative and/or semi-quantitative detection of IgG antibodies to the diffuse ( D ) and restricted ( R ) components of the EBV-EA complex in human serum.

Features

-          Highly sensitive and specific method.

-          Rapid procedure: 2x 30min.

-          Similar procedure to all Merifluor IFA kits.

-          Kit components available separately.

Specimens

Fresh, refrigerated or frozen human serum.

Kit Components

-          10-well EBV-EA substrate slides.

-          Lyophilised fluorescein-labeled  anti-human IgG conjugate.

-          Lyophilised serum diluent.

-          Lyophilised human PC and NC sera.

-          Phosphate-buffered Saline (PBS powder).

-          Buffered mounting medium.

Assay Protocol:

1.       Prepare a 1:10 screening dilution of the patient serum with prepared serum diluent. Serial dilutions can be done for title determination.

2.       Reconstitute conjugate and each control with prepared PBS.

3.       Add 1 drop (15µL) of diluted samples and control to the appropriate wells.

4.       Incubate at 37°C in a moist chamber for 30min.

5.       Rinse slides with prepared PBS solution.

6.       Add 1 drop (15µL) of conjugate to each well.

7.       Incubate at 37°C in a moist chamber for 30min.

8.       Rinse slides with prepared PBS solution.

9.       Add mounting medium and apply a cover slip on the slide.

10.   Read slide using a fluorescence microscope.

Storage

The kit must be stored at 2-8°C.

Additional Information

Click here for more information (complete procedure, safety data sheet, literature).