Catalog # HS150 – 100 tests

Description

Indirect immunofluorescence assay designed for qualitative and/or semi-quantitative detection of IgM antibodies to Herpes Simplex Virus  in human serum.

Features

-          Highly specific detection of antibodies to (1+2)-HSV.

-          Easier and quicker procedure compared to viral isolation.

-          Similar procedure to all Merifluor IFA kits.

Specimens

Fresh, refrigerated or frozen human serum.

Kit Components

-          10-well HSV Antigen substrate slides.

-          Lyophilised Fluorescein-labeled  anti-human IgM conjugate.

-          Lyophilised human PC and NC sera.

-          Phosphate-buffered Saline (PBS powder).

-          Buffered mounting medium.

Assay Protocol:

1.       Prepare a 1:10 screening dilution of the patient serum with rehydrated PBS or ready-to-use IgG inactivation reagent (Gullsorb Cat.#XX715).

2.       Reconstitute conjugate and controls with prepared PBS.

3.       Add 1 drop (15µL) of diluted samples and controls to the appropriate wells.

4.       Incubate at 37°C  in a moist chamber for 60min.

5.       Rinse slides with prepared PBS solution.

6.       Add 1 drop (15µL) of conjugate to each well.

7.       Incubate at 37°C  in a moist chamber for 30min.

8.       Rinse slides with prepared PBS solution.

9.       Add mounting medium and apply a cover slip on the slide.

10.   Read slide using a fluorescence microscope.

Storage

The kit must be stored at 2-8°C.

Additional Information

Click here for more information (complete procedure, safety data sheet, literature).